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References [ 27 ]
Marques A, Dinh T, Ioakeimidis C, Huys G, Swings J, Verstraete W, Dhont J, Sorgeloos P & Bossier P (2005) Effects of bacteria on Artemia franciscana cultured in different gnotobiotic environments. Applied and Environmental Microbiology 71: 4307-4317.
Marques A, Dhont J, Sorgeloos P & Bossier P (2004) Evaluation of different yeast cell wall mutants and microalgae strains as feed for gnotobiotically grown brine shrimp Artemia franciscana. Journal of Experimental Marine Biology and Ecology. 312: 115-136.
Chance R, Malin G, Jickells T & Baker AR (2007) Reduction of iodate to iodide by cold water diatom cultures. Marine Chemistry 105: 169-180.
Hejazi MA, Barzegari A, Gharajeh NH & Hejazi MS (2010) Introduction of a novel 18S rDNA gene arrangement along with distinct ITS region in the saline water microalga Dunaliella. Saline Systems 6: 4.
Marques A, Thanh TH, Sorgeloos P & Bossier P (2006) Use of microalgae and bacteria to enhance protection of gnotobiotic Artemia against different pathogens. Aquaculture 258: 116-126.
Alvarez LA, Exton DA, Timmis KN, Suggett DJ & McGenity TJ (2009) Characterization of marine isoprene-degrading communities. Environmental Microbiology 11: 3280-3291.
Evans C, Kadner S, Darroch LJ, Wilson WH, Liss PS & Malin G (2007) The relative significance of viral lysis and microzooplankton grazing as pathways of dimethylsulfoniopropionate (DMSP) cleavage: An Emiliania huxleyii culture study. Limnology and Oceanography 52(3): 1036-1045.
Assuncao P, Jaen-Molina R, Caujape-Castells J, de la Jara A, Carmona L, Freijanes K & Mendoza H (2011) Phylogenetic position of Dunaliella acidophila (Chlorophyceae) based on ITS and rbcL sequences. Journal of Applied Phycology 24: 635-639.
Roleda MY, Slocombe SP, Leakey RJG, Day JG, Bell EM & Stanley MS (2013) Effects of temperature and nutrient regimes on biomass and lipid production by six oleaginous microalgae in batch culture employing a two-phase cultivation strategy. Bioresource Technology 129: 439-449.
Pei S & Laws EA (2013) Does the 14C method estimate net photosynthesis? Implications from batch and continuous culture studies of marine phytoplankton. Deep-Sea Research I 82: 1-9.
Assuncao P, Jaen-Molina R, Caujape-Castells J, de la Jara A, Carmona L, Freijanes K & Mendoza H (2012) Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling. Aquatic Biosystems 8: 2.
DOI: none
Toi HT, Boeckx P, Sorgeloos P, Bossier P & Van Stappen G (2014) Co-feeding of microalgae and bacteria may result in increased N assimilation in Artemia as compared to mono-diets, as demonstrated by a 15N isotope uptake laboratory study. Aquaculture 422-423: 109-114.
Pretti C, Oliva M, Di Pietro R, Monni G, Cevasco G, Chiellini F, Pomelli C & Chiappe C (2014) Ecotoxicity of pristine graphene to marine organisms. Ecotoxicology and Environmental Safety 101: 138-145.
Sjollema SB, van Beusekom SAM, van der Geest HG, Booij P, de Zwart D, Vethaak AD & Admiraal W (2014) Laboratory algal bioassays using PAM fluorometry: Effects of test conditions on the determination of herbicide and field sample toxicity. Environmental Toxicology and Chemistry 33: 1017-1022.
Sjollema SB, Martinez Garcia G, van der Geest HG, Kraak MHS, Booij P, Vethaak AD & Admiraal W (2014) Hazard and risk of herbicides for marine microalgae. Environmental Pollution 187: 106-111.
Emami K, Hack E, Nelson A, Brain CM, Lyne FM, Mesbahi E, Day JG & Caldwell GS (2015) Proteomic-based biotyping reveals hidden diversity within a microalgae culture collection: An example using Dunaliella. Scientific Reports 5: 10036.
Day JG, Burt DJ, Achilles-Day UEM & Stanley MS (2013) Future algal biofuels: Implications of environmental temperature on production strain selection. International Journal of Ambient Energy 36: 248-252.
Kumar A, Pathak AK & Guria C (2015) NPK-10:26:26 complex fertilizer assisted optimal cultivation of Dunaliella tertiolecta using response surface methodology and genetic algorithm. Bioresource Technology 194: 117-129.
Slocombe SP, Zhang QY, Ross M, Anderson A, Thomas NJ, Lapresa A, Rad Menéndez C, Campbell CN, Black KD, Stanley MS & Day JG (2015) Unlocking nature's treasure-chest: Screening for oleaginous algae. Scientific Reports 5: 09844.
Zhou C, Vitiello V, Pellegrini D, Wu C, Morelli E & Buttino I (2016) Toxicological effects of CdSe/ZnS quantum dots on marine planktonic organisms. Ecotoxicology and Environmental Safety 123: 26-31.
Sjollema SB, Redondo-Hasselerharm P, Leslie HA, Kraak MHS & Vethaak AD (2016) Do plastic particles affect microalgal photosynthesis and growth? Aquatic Toxicology 170: 259-261.
Kumar A, Guria C, Chitres G, Chakraborty A & Pathak AK (2016) Modelling of microalgal growth and lipid production in Dunaliella tertiolecta using nitrogen-phosphorus-potassium fertilizer medium in sintered disk chromatographic glass bubble column. Bioresource Technology 218: 1021-1036.
Sinha SK, Kumar M, Guria C, Kumar A & Banerjee C (2017) Biokinetic model-based multi-objective optimization of Dunaliella tertiolecta cultivation using elitist non-dominated sorting genetic algorithm with inheritance. Bioresource Technology -: -.
Kumar A, Guria C & Pathak AK (2017) Potential CO2 fixation and optimal Dunaliella tertiolecta cultivation: Influence of fertilizer, wavelength of light-emitting diodes, salinity and carbon supply strategy Journal of CO2 Utilization 22: 164-177.
Kumar A, Guria C & Pathak A (2018) Optimal cultivation towards enhanced algae-biomass and lipid production using Dunaliella tertiolecta for biofuel application and potential CO2 bio-fixation: Effect of nitrogen deficient fertilizer, light intensity, salinity and carbon supply strategy Energy 148: 1069-1086.
Oliveira Bezerra da Silva MR, Santana Moura YA, Converti A, Figueiredo Porto AL, Viana Marques DA & Bezerra RP (2021) Assessment of the potential of Dunaliella microalgae for different biotechnological applications: A systematic review Algal Research 58: 102396.
Parsy A, Monlau F, Guyoneaud R & Sambusiti C (2024) Nutrient recovery in effluents from the energy sectors for microalgae and cyanobacteria biomass production: A review Renewable and Sustainable Energy Reviews 191: 114207.
Sequences [ 5 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
SSU (18S)
Division/Phylum: Chlorophyta Class: Chlorophyceae Order: Chlamydomonadales

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: f/2; Minimal or unobserved bacteria under normal growth conditions; maintained by serial subculture and cryopreserved;
AuthorityButcher 1959
IsolatorUnknown (pre 1984)
Collection Site Halifax, Canada
Notes Received by CCAP in 1984 from CSIRO, acquired by CSIRO in 1964
Axenicity Status Minimal or unobserved bacteria under normal growth conditions
Area North America
Country Canada
Environment Unknown
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date pre 1984
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet SM_AquacultureStrains.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID 178590
Equivalent StrainsCSIRO CS-14c

CCAP 19/27

Dunaliella tertiolecta

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