References [ 3 ]
Brown S & Smirnov AV (2004) Diversity of gymnamoebae in grassland soil in southern Scotland Protistology 3: 191-195.
DOI: none
Bass D, Silberman JD, Brown MW, Pearce RA, Tice AK, Jousset A, Geisen S & Hartikainen H (2016) Coprophilic amoebae and flagellates, including Guttulinopsis, Rosculus and Helkesimastix, characterise a divergent and diverse rhizarian radiation and contribute to a large diversity of faecal-associated protists. Environmental Microbiology 18: 1604-1619.
Schuler GA, Tice AK, Pearce RA, Foreman E, Stone J, Gammill S, Willson JD, Reading C, Silberman JD & Brown MW (2018) Phylogeny and Classification of Novel Diversity in Sainouroidea (Cercozoa, Rhizaria) Sheds Light on a Highly Diverse and Divergent Clade Protist 169: 853-874.
Division/Phylum: Discoba Class: Heterolobosea Order: Schizopyrenida
Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology
and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee
that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive
from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and
Adl et al. (2019) for protists.
Culture media, purity and growth conditions:
Medium:
NN;
Bacteria present; maintained by serial subculture; 15-20 deg C; sub 8-12 weeks; contains non-pathogenic E. coli as food source
| Attributes | |
| Authority | Hawes 1963 |
| Isolator | Brown (1999) |
| Collection Site | Block 1, Plot C Rigg Foot Experimental Site, Sourhope field, (Yetholm, Kelsoe), Cheviots, Scotland, UK |
| Climatic Zone | Temperate |
| Notes | Isolation: incubation of diluted soil sample in PAS in multiwell plates (and nutrient agar blocks) followed by plating out on NN agar and E. coli; Cystformer; Can be successfully cryopreserved - a user grew it successfully on weak Malt-Yeast agar plates (wMY) streaked with Klebsiella: "let the amoeba eat through most of the bacteria. The plate was then flooded with 3 ml of Pages Amoeba Saline (PAS), the agar surface was then scrapped to detach the amoebae and the liquid collected (PAS + amoeba + residual bacteria) into 2x 1.7ml microcentrifuge tubes. The cells were pelleted at 5000xg for 5 min. The supernatant was aspirated and discarded. The cell pellets were combined in a total of 1.1 ml of PAS and placed on ice. An equal volume of PAS + 14% DMSO was added to the cells and mixed by inversion (bringing the final concentration of DMSO to 7%). 0.75 ml aloquotes were distributed into 3 cryovials and control-cooled overnight to -80°C in a "mister frosty". The vials were then placed in liquid nitrogen. To recover Rosculus, a frozen vial was thawed by placing it at 37°C in a water bath for ~2min (until the contents are just thawed... do not let the the contents warm past room temp.). One drop of the contents was place at the end of a bacterial streak (Klebsiella) on a wMY agar plate. The cells recovered great and were eating up the streak of bacteria within 3 days." |
| Axenicity Status | Bacteria present |
| Area | Europe |
| Country | UK |
| Environment | Soil |
| GMO | No |
| Group | Protozoa |
| In Scope of Nagoya Protocol | No |
| ABS Note | Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain. |
| Collection Date | c 1999 |
| Original Designation | 2/1Cb/2A |
| Pathogen | Not pathogenic: Hazard Class 1 |
| Strain Maintenance Sheet | SM_Rosculus.pdf |
| Toxin Producer | Not Toxic / No Data |
| Type Culture | No |
| Taxonomy WoRMS ID | |