References [ 5 ]
Costas M & Griffiths AJ (1984) The esterases and acid-phosphatases of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 33-41.
DOI: none
Daggett PM, Sawyer T & Nerad T (1982) Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microbial Ecology 8(4): 371-386.
Kong, HH, Shin JY, Yu HS, Kim J, Hahn TW, Hahn YH & Chung DI (2002) Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea Journal of Clinical Microbiology 40: 1199-1206.
Reveiller FL, Marciano-Cabral F, Pernin P, Cabanes PA & Legastelois S (2000) Species specificity of a monoclonal antibody produced to Naegleria fowleri and partial characterisation of its antigenic determinant. Parasitology Research 86: 634-641.
DOI: none
Martinez AJ, Markowitz SM & Duma RJ (1975) Experimental pneumonitis and encephalitis caused by Acanthamoeba in mice: pathogenesis and ultrastructural features. The Journal of Infectious Diseases 131(6): 692-699.
Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: NN; Monoxenic; maintained by serial subculture and cryopreserved; contains non-pathogenic E. coli as food source.
Attributes
AuthoritySawyer, Visvesvera & Harke 1977
IsolatorChang (1959)
Collection Siteprivate well water nr. Cincinnati, Ohio, USA
Notes Originally identified by isolator as Hartmanella glebae, deposited as A. castellanii, re-identified as A. hatchetti in 1994 based on RFLP analysis of mitochondrial DNA
Axenicity Status Monoxenic
Area North America
Country USA
Environment Freshwater
GMO No
Group Protozoa
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date 1959
Pathogen Potential Pathogen: Hazard Class 2
Strain Maintenance Sheet
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID
Equivalent StrainsATCC 30898
Formerly Listed in CCAP asAcanthamoeba castellanii (Douglas) Volkonsky 1931

CCAP 1501/2B

Acanthamoeba hatchetti

  • Product Code: CCAP 1501/2B
  • Availability: Potential Pathogen
You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.