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Gião MS, Wilks SA, Azevedo NF, Vieira MJ & Keevil CW (2009) Validation of SYTO 9/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophila Microbial Ecology 58: 56-62.

DOI: 10.1007/s00248-008-9472-x

Lorenzo-Morales J, Ortega-Rivas A, Foronda P, Martínez E & Valladares B (2005) Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources. Parasitology Research 95: 273-277.

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Mura M, Bull TJ, Evans H, Sidi-Boumedine K, McMinn L, Rhodes G, Pickup R & Hermon-Taylor J (2006) Replication and long-term persistence of bovine and human strains of Mycobacterium avium subsp. paratuberculosis within Acanthamoeba polyphaga. Applied and Environmental Microbiology 72: 854-859.

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Lorenzo-Morales J, Ortega-Rivas A, Foronda P, Abreu-Acosta N, Ballart D, Martínez E & Valladares B (2005) RNA interference (RNAi) for the silencing of extracellular serine proteases genes in Acanthamoeba: Molecular analysis and effect on pathogenicity. Molecular and Biochemical Parasitology 144: 10-15.

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Ortega-Rivas A, Lorenzo-Morales J, Martínez E, Villa M, Clavel A, Valladares B & del Castillo A (2005) A specific primer pair for the diagnosis and identification of Acanthamoeba astronyxis by random amplified polymorphic DNA-polymerase chain reaction. Journal of Parasitology 91: 122-126.

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Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:

Medium: NN; Monoxenic; maintained by serial subculture;

Attributes
Authority	(Puschkarew) Volkonsky 1931
Isolator	Rowbotham (1985)
Collection Site	cooling tower Lincoln, England, UK
Axenicity Status	Monoxenic
Area	Europe
Country	UK
Environment	Freshwater
GMO	No
Group	Protozoa
In Scope of Nagoya Protocol	No
ABS Note	Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date	c 1985
Original Designation	Linc Ap-1
Pathogen	Potential Pathogen: Hazard Class 2
Strain Maintenance Sheet
Toxin Producer	Not Toxic / No Data
Type Culture	No
Taxonomy WoRMS ID	163677

CCAP 1501/18

Acanthamoeba polyphaga

Product Code: CCAP 1501/18
Availability: Potential Pathogen

You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.

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