References [ 10 ]
Snelling WJ, McKenna JP, Lecky DM & Dooley JSG (2005) Survival of Campylobacter jejuni in waterborne protozoa. Applied and Environmental Microbiology 71: 5560-5571.
Zhang J, Laakso J, Mappes J, Laanto E, Ketola T, Bamford JKH, Kunttu H & Sundberg L (2014) Association of colony morphotypes with virulence, growth and resistance against protozoan predation in the fish pathogen Flavobacterium columnare. FEMS Microbiology Ecology 89: 553-562.
Coulon C, Collignon A, McDonnell G & Thomas V (2010) Resistance of Acanthamoeba cysts to disinfection treatments used in health care settings. Journal of Clinical Microbiology 48: 2689-2697.
Ovrutsky AR, Chan ED, Kartalija M, Bai X, Jackson M, Gibbs S, Falkinham III JO, Iseman MD, Reynolds PR, McDonnell G & Thomas V (2013) Cooccurrence of free-living amoebae and nontuberculous mycobacteria in hospital water networks, and preferential growth of Mycobacterium avium in Acanthamoeba lenticulata. Applied and Environmental Microbiology 79: 3185-3192.
Zhang J, Ketola T, Örmälä-Odegrip A, Mappes J & Laakso J (2014) Coincidental loss of bacterial virulence in multi-enemy microbial communities. PLoS ONE 9: e111871.
Fuerst PA, Booton GC & Crary M (2014) Phylogenetic analysis and the evolution of the 18S rRNA gene typing system of Acanthamoeba. Journal of Eukaryotic Microbiology 62: 69-84.
Zhang J, Ormala-Odegrip A, Mappes J & Laakso J (2014) Top-down effects of a lytic bacteriophage and protozoa on bacteria in aqueous and biofilm phases. Ecology and Evolution 4: 4444-4453.
Vieira A, Seddon AM & Karlyshev AV (2015) Campylobacter - Acanthamoeba interactions. Microbiology 161: 933-947.
Fuerst, PA (2023) The status of molecular analyses of isolates of Acanthamoeba maintained by international culture collections. Microorganisms 11(2): 295.
Fuerst PA & Booton GC (2020) Species, sequence types and alleles: Dissecting genetic variation in Acanthamoeba Pathogens 9(7): 534.
Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: NN; Monoxenic; maintained by serial subculture and cryopreserved; contains non-pathogenic E. coli as food source.
Attributes
Authority(Douglas) Volkonsky 1931
IsolatorCastellani (1930)
Collection SiteCryptococcus pararoseus yeast culture England
Axenicity Status Monoxenic
Area Europe
Country UK
Environment Freshwater
GMO No
Group Protozoa
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date c 1930
Pathogen Potential Pathogen: Hazard Class 2
Strain Maintenance Sheet
Toxin Producer Not Toxic / No Data
Type Culture Yes
Taxonomy WoRMS ID

CCAP 1501/10

Acanthamoeba castellanii

  • Product Code: CCAP 1501/10
  • Availability: Potential Pathogen
You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.