References [ 13 ]
Gachon CMM, Day JG, Campbell CN, Pröschold T, Saxon RJ & Küpper FC (2007) The Culture Collection of Algae and Protozoa (CCAP): A biological resource for protistan genomics Gene 406: 51-57.
Krienitz L, Bock C, Luo W & Pröschold T (2010) Polyphyletic origin of the Dictyosphaerium morphotype within Chlorellaceae (Trebouxiophyceae). Journal of Phycology 46: 559-563.
Luo W, Pröschold T, Bock C & Krienitz L (2010) Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae). Plant Biology 12: 545-553.
Pröschold T, Darienko T, Silva PC, Reisser W & Krienitz L (2011) The systematics of Zoochlorella revisited employing an integrative approach. Environmental Microbiology 13: 350-364.
Ducruet J-M, Serrano A, Roncel M & Ortega JM (2011) Perculiar properties of chlorophyll thermoluminescence emission of autotrophically or mixotrophically grown Chlamydomonas reinhardtii. Journal of Photochemistry and Photobiology B: Biology 104: 301-307.
Hoshina R, Iwataki M & Imamura N (2010) Chlorella variabilis and Micractinium reisseri sp. nov. (Chlorellaceae, Trebouxiophyceae): Redescription of the endosymbiotic green algae of Paramecium bursaria (Peniculia, Oligohymenophorea) in the 120th year. Phycological Research 58: 188-201.
Krienitz L, Bock C, Kotut K & Proschold T (2012) Genotypic diversity of Dictyosphaerium-morphospecies (Chlorellaceae, Trebouxiophyceae) in African inland waters, including the description of four new genera. Fottea 12: 231-253.
DOI: none
Bock C, Krienitz L & Proschold T (2011) Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species. Fottea 11: 293-312.
DOI: none
Omairi T & Wainwright M (2015) Fluorescent minerals - A potential source of UV protection and visible light for the growth of green algae and cyanobacteria in extreme cosmic environments. Life Sciences in Space Research 6: 87-91.
Mthakathi NT, Kgosiemang IKR, Chen W, Mohlatsane ME, Mojahi TJ, Yu JH, Mashele SS & Syed K (2015) Cytochrome P450 monooxygenase analysis in free-living and symbiotic microalgae Coccomyxa sp. C-169 and Chlorella sp. NC64A Algae 30: 233-239.
Higuchi R, Song C, Hoshina R & Suzaki T (2018) Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea) European Journal of Protistology 66: 149-155.
Loganathan BG, Orsat V & Lefsrud M (2020) Phycoremediation and valorization of synthetic dairy wastewater using microalgal consortia of Chlorella variabilis and Scenedesmus obliquus Environmental Technology -: -.
Spanner C, Darienko T, Biehler T, Sonntag B & Pröschold T (2020) Endosymbiotic green algae in Paramecium bursaria: A new isolation method and a simple diagnostic PCR approach for the identification Diversity 12: 240.
Sequences [ 3 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
Whole Genome Shotgun (WGS)
Division/Phylum: Chlorophyta Class: Trebouxiophyceae Order: Chlorellales

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: EG:JM; Axenic; maintained by serial subculture;
Attributes
AuthorityShihira & Krauss 1965
IsolatorVan Etten
Collection Siteendosymbiont of Paramecium bursaria CCAP 1660/25 North Carolina, USA
Notes syngen 1; endosymbiont of Paramecium bursaria strain CCAP 1660/25
Axenicity Status Axenic
Area North America
Country USA
Environment Freshwater
GMO No
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date pre 2007
Original Designation NC64A
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet SM_GeneralFreshwaterGreens.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID 700795
Other DesignationsATCC-50258
Formerly Listed in CCAP asChlorella sp.

CCAP 211/84

Chlorella variabilis


Related Products

EG:JM Medium<br>CONCENTRATED STOCKS

CCAP FAEGJM-C
EG:JM Medium
CONCENTRATED STOCKS

Non-sterile concentrated stocks to make up 5 litres of EG:JM medium (a 1:1 mix of EG and JM media).

EG:JM Medium<br>1 LITRE PREMADE

CCAP FAEGJM-P
EG:JM Medium
1 LITRE PREMADE

1 litre of sterile, ready to use, EG:JM medium. EG:JM medium is used for culturing freshwater algae