{"id":473,"date":"2020-06-17T14:59:12","date_gmt":"2020-06-17T13:59:12","guid":{"rendered":"https:\/\/ccap2020.sams.local\/?page_id=473"},"modified":"2025-12-17T13:25:17","modified_gmt":"2025-12-17T13:25:17","slug":"knowledgebase","status":"publish","type":"page","link":"https:\/\/www.ccap.ac.uk\/index.php\/knowledgebase\/","title":{"rendered":"Knowledgebase"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"473\" class=\"elementor elementor-473\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-962949f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"962949f\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e0d4d68\" data-id=\"e0d4d68\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-50afbee elementor-widget elementor-widget-heading\" data-id=\"50afbee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">CCAP Knowledgebase<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4582ec6 elementor-widget elementor-widget-text-editor\" data-id=\"4582ec6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<img decoding=\"async\" class=\"wp-image-726 size-thumbnail alignleft\" src=\"\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-150x150.jpg\" alt=\"\" width=\"150\" height=\"150\" srcset=\"https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-150x150.jpg 150w, https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-300x300.jpg 300w, https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-1024x1024.jpg 1024w, https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-768x768.jpg 768w, https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-1536x1536.jpg 1536w, https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-270x270.jpg 270w, https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop-230x230.jpg 230w, https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/2020\/09\/CCAP-6038_1-crop.jpg 1754w\" sizes=\"(max-width: 150px) 100vw, 150px\" \/>CCAP has accumulated a wealth of resources about the strains we maintain, ranging from information on their provenance, their properties, images, genetic sequences and references from the scientific literature where the strains have been used. Accessible too from this page are methods relating to strain culture, isolation, cryopreservation and media recipes. More will be added as the resource continues to grow.\n\nWe are starting to produce and upload short videos demonstrating some of the methods and techniques we use.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3f74dff elementor-widget elementor-widget-text-editor\" data-id=\"3f74dff\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Culture Media<\/h2>\n<ul>\n \t<li><a href=\"https:\/\/www.ccap.ac.uk\/index.php\/media-recipes\/\">List of basic media used at CCAP with recipes<\/a><\/li>\n \t<li><!--<a href=\"https:\/\/www.ccap.ac.uk\/index.php\/media-supply\/\">Media we supply<\/a> and our --><a href=\"https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/KB_CCAP_Media_Supply_Factsheet.pdf\" target=\"_blank\" rel=\"noopener\">Information on using and storing media supplied by CCAP (stocks or premade)<\/a>.<\/li>\n \t<li>General <a href=\"https:\/\/www.ccap.ac.uk\/wp-content\/uploads\/KB_CCAPCultureMediaFactsheet.pdf\" target=\"_blank\" rel=\"noopener\">factsheet on preparing culture media<\/a><\/li>\n<\/ul>\n<a href=\"https:\/\/mediadive.dsmz.de\/\" target=\"_blank\" rel=\"noopener\">Media<em>Dive<\/em><\/a>: media recipes used to grow many of our algae and protozoa have been added to the <a href=\"https:\/\/mediadive.dsmz.de\/\" target=\"_blank\" rel=\"noopener\">Media<em>Dive<\/em> <\/a>database developed at the DSMZ &#8211; the world&#8217;s largest collection of cultivation media for bacteria and now algae and protozoa.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-40b962f elementor-widget elementor-widget-heading\" data-id=\"40b962f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Methods<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7054c5b elementor-widget elementor-widget-html\" data-id=\"7054c5b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<script>\r\njQuery(document).ready(function($) {\r\nvar delay = 100; setTimeout(function() {\r\n$('.elementor-tab-title').removeClass('elementor-active');\r\n $('.elementor-tab-content').css('display', 'none'); }, delay);\r\n});\r\n<\/script>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1b95bec elementor-widget elementor-widget-accordion\" data-id=\"1b95bec\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"accordion.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2891\" class=\"elementor-tab-title\" data-tab=\"1\" role=\"button\" aria-controls=\"elementor-tab-content-2891\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><i class=\"fas fa-chevron-down\"><\/i><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><i class=\"fas fa-chevron-up\"><\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Culture Maintenance<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2891\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"region\" aria-labelledby=\"elementor-tab-title-2891\"><p>Short videos demonstrating some of our subculturing techniques:<\/p><ul><li><a href=\"https:\/\/vimeo.com\/776274749\" target=\"_blank\" rel=\"noopener\">Subculturing amoebae using block transfer method<\/a><\/li><li><a href=\"https:\/\/vimeo.com\/776274922\" target=\"_blank\" rel=\"noopener\">Subculturing amoebae using wash method<\/a><\/li><li><a href=\"https:\/\/vimeo.com\/776274800\" target=\"_blank\" rel=\"noopener\">Subculturing cyst-forming amoebae<\/a><\/li><li><a href=\"https:\/\/vimeo.com\/777015744\" target=\"_blank\" rel=\"noopener\">Subculturing small seaweeds<\/a><\/li><\/ul><p>General culturing information:<\/p><p>Algae<\/p><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_PyrocystisCulturing.pdf\" target=\"_blank\" rel=\"noopener\"><em>Pyrocystis lunula<\/em><\/a><\/li><\/ul><p>Protozoa<\/p><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Amoeba_marine_maintenance.pdf\" target=\"_blank\" rel=\"noopener\">Marine amoebae<\/a><\/li><li><a href=\"https:\/\/www.ccap-test.ac.uk\/wp-content\/uploads\/2020\/06\/KB_Ciliate_marine_maintenance.pdf\" target=\"_blank\" rel=\"noopener\">Marine ciliates<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Flagellate_marine_maintenance.pdf\" target=\"_blank\" rel=\"noopener\">Marine heterotrophic flagellate protists<\/a><\/li><\/ul><p>Other<\/p><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Tetrahymena_washing.pdf\" target=\"_blank\" rel=\"noopener\">Washing <em>Tetrahymena pyriformis<\/em> for feeding to protists<\/a><\/li><\/ul><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2892\" class=\"elementor-tab-title\" data-tab=\"2\" role=\"button\" aria-controls=\"elementor-tab-content-2892\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><i class=\"fas fa-chevron-down\"><\/i><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><i class=\"fas fa-chevron-up\"><\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Cryopreservation<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2892\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"region\" aria-labelledby=\"elementor-tab-title-2892\"><p>We have protocols for both a passive freezer (e.g. Mr Frosty) or a controlled rate cooler:<\/p><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_MrFrosty_cryo.pdf\" target=\"_blank\" rel=\"noopener\">Using a passive freezer<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Controlled_rate_cooling_cryo.pdf\" target=\"_blank\" rel=\"noopener\">Using a controlled rate cooler<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Ectocarpus_cryo_protocol.pdf\" target=\"_blank\" rel=\"noopener\">Cryopreserving Ectocarpus<\/a><\/li><\/ul><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2893\" class=\"elementor-tab-title\" data-tab=\"3\" role=\"button\" aria-controls=\"elementor-tab-content-2893\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><i class=\"fas fa-chevron-down\"><\/i><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><i class=\"fas fa-chevron-up\"><\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Strain Isolation and purification<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2893\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"3\" role=\"region\" aria-labelledby=\"elementor-tab-title-2893\"><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Isolation_tips.pdf\" target=\"_blank\" rel=\"noopener\">Physical isolation<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Antibiotic_treatment.pdf\" target=\"_blank\" rel=\"noopener\">Antibiotic treatment<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Endosymbiont_isolation.pdf\" target=\"_blank\" rel=\"noopener\">Isolating symbionts<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Fungus_removal_physical_tips_SAMS.pdf\" target=\"_blank\" rel=\"noopener\">Physical removal of fungus<\/a><\/li><\/ul><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2894\" class=\"elementor-tab-title\" data-tab=\"4\" role=\"button\" aria-controls=\"elementor-tab-content-2894\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><i class=\"fas fa-chevron-down\"><\/i><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><i class=\"fas fa-chevron-up\"><\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Molecular<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2894\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"4\" role=\"region\" aria-labelledby=\"elementor-tab-title-2894\"><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Barcoding-protocol-Assemble-Updated_0518.pdf\" target=\"_blank\" rel=\"noopener\">Barcoding<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_AFLP_protocol.pdf\" target=\"_blank\" rel=\"noopener\">AFLP analysis<\/a><\/li><\/ul><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2895\" class=\"elementor-tab-title\" data-tab=\"5\" role=\"button\" aria-controls=\"elementor-tab-content-2895\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><i class=\"fas fa-chevron-down\"><\/i><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><i class=\"fas fa-chevron-up\"><\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Enumeration<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2895\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"5\" role=\"region\" aria-labelledby=\"elementor-tab-title-2895\"><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_FlowCam_protocol.pdf\">Using the Flow-Cam<\/a><\/li><\/ul><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-2896\" class=\"elementor-tab-title\" data-tab=\"6\" role=\"button\" aria-controls=\"elementor-tab-content-2896\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><i class=\"fas fa-chevron-down\"><\/i><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><i class=\"fas fa-chevron-up\"><\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Medium Scale Culture<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-2896\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"6\" role=\"region\" aria-labelledby=\"elementor-tab-title-2896\"><ul><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Medium_scale_culture.pdf\" target=\"_blank\" rel=\"noopener\">Polycarbonate carboys<\/a><\/li><li><a href=\"\/wp-content\/uploads\/2020\/06\/KB_Medium_scale_plastic.pdf\" target=\"_blank\" rel=\"noopener\">Plastic bag cultures<\/a><\/li><\/ul><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>CCAP Knowledgebase CCAP has accumulated a wealth of resources about the strains we maintain, ranging from information on their provenance, their properties, images, genetic sequences and references from the scientific literature where the strains have been used. Accessible too from<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-473","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/pages\/473","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/comments?post=473"}],"version-history":[{"count":73,"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/pages\/473\/revisions"}],"predecessor-version":[{"id":3711,"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/pages\/473\/revisions\/3711"}],"wp:attachment":[{"href":"https:\/\/www.ccap.ac.uk\/index.php\/wp-json\/wp\/v2\/media?parent=473"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}