Name: S/W (Soil/Water Biphasic Medium)

Description: Protozoa

Medium
Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is seldom satisfactory.) Carefully add deionized water to a depth of 7 to 10 cm, plug or cover, and steam for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on each of 2 consecutive days; further sterilization is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7.0 - 8.0.

Ref Pringsheim, E.G. (1946) The biphasic or soil-water culture method of growing algae or flagellata. 

       J. Ecol., 33: 193-204