recipe

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Name: A/LCB (Bourrelly Medium; modified)

Description: Freshwater algae and protozoa

Stock solutions per 100 ml distilled water
(1) KNO3 - 10.0 g
(2) K2HPO4 - 1.0 g
(3) MgSO4.7H2O - 1.0 g
(4) Ca(NO3)2 - 1.0 g
(5) Vitamin solution

      Biotin - 4.0 mg

      B12 - 4.0 mg

      B1 - 4.0 mg
(6) FeSO4.7H2O - 0.7 g

      EDTANa2 - 0.93 g

(7) Trace metal solution
  Make up individual solutions (g per 100 ml distilled water) then make up the stock solution using the quantities in the second column and adding distilled water to 100 ml.
MnCl2.4H2O 0.99 g 1.0 ml
CoSO4.7H2O 0.28 g 0.1 ml
CuSO4.5H2O 0.05 g 0.1 ml
ZnSO4.7H2O 0.315 g 0.2 ml
H3BO3 0.62 g 0.5 ml
(NH4)6Mo7O24.2H2O 0.18 g 0.1 ml
NiSO4.6H2O 0.263 g 1.0 ml
NH4VO3 0.146 g 0.1 ml

Medium per litre

Stock solutions 1 - 4 , 1.0 ml each

Stock solution 5 - 10.0 ml

Stock solution 6 - 7 - 0.5 ml each
Na2CO3 - 0.3 g

NaCl - 15.0 g

Method

Make up to 1 litre with distilled water.  For agar add 15.0 g per litre of *Bacteriological Agar

(Oxoid L11).  Autoclave at 15 psi for 15 minutes.

Supply *Unipath Ltd, Wade Road, Basingstoke, Hants, RG24 0PW, UK


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