Name: ABM (Acidic Bold-Basal Medium with Vitamins; modified)

Description: Freshwater algae

Dissolve 250 mg (NH4)2SO4 in 800 ml distilled water and add the following:

Stocks per litre
(1) NaNO3 - 25.0 g
(2) MgSO4.7H2O - 7.5 g
(3) NaCl - 2.5 g
(4) K2HPO4.3H2O - 7.5 g
(5) KH2PO4 - 17.5 g
(6) CaCl2.2H2O - 2.5 g

(7) Trace elements solution

      Add to 1000 ml of distilled water 0.75 g Na2-EDTA and these minerals exactly in the following sequence:
      FeCl3.6H2O - 97.0 mg

      MnCl2.4H2O - 41.0 mg

      ZnCl2.6H2O - 5.0 mg

      CoCl2.6H2O - 2.0 mg

      Na2MoO4.2H2O - 4.0 mg

(8) Vitamin B1

       0.12 g Thiaminhydrochloride in 100 ml distilled water and filtered sterile

(9) Vitamin B12

       0.1 g Cyanocobalamin in 100 ml distilled water, take 1 ml of this solution and add 99 ml distilled water, filter sterile.

Medium

Stock solutions 1 - 6, 10.0 ml each
Stock solution 7, 6.0 ml

Stock solutions 8 - 9, 1.0 ml each

Method

Make up to 1 litre with deionized water and adjust the pH to 3.0 with HCl.  Autoclave at 15 psi for 15 minutes.

Reference
Pollio A, Cennamo P, Ciniglia C, De Stefano M, Pinto G & Huss VAR (2005) Chlamydomonas pitschmanii Ettl, a Little Known Species from Thermoacidic

Environments. Protist 156: 257-302.